Long Non-Coding RNA OIP5-AS1 Inhibits the Proliferation and Migration of Esophageal Squamous Carcinoma Cells by Targeting FOXD1/miR-30a-5p Axis and the Effect of Micro- and Nano-Particles on Targeting Transfection System
| Autor: | Zixiang Wu, Ming Wu, Chuanqiang Wu, Qihang Yan, Li Liu, Lingjun Dong, Qi Wang, Zhen Wang, Chendi Xu, Jun Ye Wang, Han Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Esophageal Neoplasms
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Transfection Cell Line Tumor Humans General Materials Science Cell Proliferation Regulation of gene expression Messenger RNA Cell growth Chemistry RNA Forkhead Transcription Factors Squamous carcinoma Gene Expression Regulation Neoplastic MicroRNAs Head and Neck Neoplasms Cell culture Cancer research RNA Long Noncoding Esophageal Squamous Cell Carcinoma Signal transduction |
| Zdroj: | Journal of Biomedical Nanotechnology. 17:1380-1391 |
| ISSN: | 1550-7033 |
| Popis: | Esophageal cancer is one of the most common human malignancies and ranks sixth for global mortality; the major histological type is esophageal squamous cell carcinoma (ESCC). Here we assessed the effect of long non-coding (lnc) RNA OIP5-AS1 on the miR-30a-5p/Forkhead box protein D1 (FOXD1) axis in ESCC and investigated the underlying mechanism involving the ERK1/2 signaling pathway. lnc RNA OIP5-AS1 was highly expressed in human ESCC tissues and cells, targeted miR-30a-5p, and inhibited miR-30a-5p expression. Additionally, in human ESCC tissues, miR-30a-5p was poorly expressed, whereas FOXD1 mRNA and protein were highly expressed, with a negative correlation between miR-30a-5p and FOXD1 expression. miR-30a-5p targeted and inhibited FOXD1 expression. FOXD1 promoted the proliferation and invasion of ESCC and was related to the ERK1/2 signaling pathway; ERK1/2 inhibitors (LY-3214996) reversed the biological function of FOXD1. miR-30a-5p combined with FOXD1 regulated ERK1/2 expression and inhibited tumor growth in vivo. In this study, micro- and nano-particles were used as carriers to construct Nanocapsules carrying miR-30a-5p mimics and miR-30a-5p inhibitor through self-assembly method, so as to realize an efficient Nanocapsules delivery system of miR-30a-5p to esophageal cancer cells. It provides insights into targeted drug therapy and the development of micro- and nano-particles carriers. |
| Databáze: | OpenAIRE |
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