Bacterial community structure and predicted alginate metabolic pathway in an alginate-degrading bacterial consortium
| Autor: | Naomichi Nishio, Tsunehiro Aki, Akihisa Kita, Yoshiko Okamura, Yukihiko Matsumura, Takahisa Tajima, Junichi Kato, Toyokazu Miura, Satoshi Kawata, Takeshi Yamaguchi, Yutaka Nakashimada |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Alginates Bioengineering medicine.disease_cause Gram-Positive Bacteria 01 natural sciences Applied Microbiology and Biotechnology Microbiology Agar plate 03 medical and health sciences Bacteria Anaerobic Clostridium Glucuronic Acid 010608 biotechnology RNA Ribosomal 16S medicine Clostridiaceae Microbial biodegradation Symbiosis biology Bacteroidetes Hexuronic Acids biology.organism_classification Metabolic pathway 030104 developmental biology Dysgonomonas capnocytophagoides Fermentation Metagenomics Bacteria Metabolic Networks and Pathways Biotechnology |
| Zdroj: | ResearcherID |
| ISSN: | 1347-4421 |
| Popis: | Methane fermentation is one of the effective approaches for utilization of brown algae; however, this process is limited by the microbial capability to degrade alginate, a main polysaccharide found in these algae. Despite its potential, little is known about anaerobic microbial degradation of alginate. Here we constructed a bacterial consortium able to anaerobically degrade alginate. Taxonomic classification of 16S rRNA gene, based on high-throughput sequencing data, revealed that this consortium included two dominant strains, designated HUA-1 and HUA-2; these strains were related to Clostridiaceae bacterium SK082 (99%) and Dysgonomonas capnocytophagoides (95%), respectively. Alginate lyase activity and metagenomic analyses, based on high-throughput sequencing data, revealed that this bacterial consortium possessed putative genes related to a predicted alginate metabolic pathway. However, HUA-1 and 2 did not grow on agar medium with alginate by using roll-tube method, suggesting the existence of bacterial interactions like symbiosis for anaerobic alginate degradation. |
| Databáze: | OpenAIRE |
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