Media conditioned by cultured human vascular endothelial cells inhibit the growth of vascular smooth muscle cells
| Autor: | M.D. Gonsalvez, Ph.G. De Groot, M.C. Janssen, J. A. Van Mourik, Wim P. Zeijlemaker, Ch Willems, Giulia C.B. Astaldi, W.G. van Aken |
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| Rok vydání: | 1982 |
| Předmět: |
Hot Temperature
Lysis Vascular smooth muscle Endothelium Biology Muscle Smooth Vascular medicine Protein biosynthesis Chymotrypsin Humans Trypsin Cells Cultured Contact Inhibition Cell growth Contact inhibition DNA Cell Biology Growth Inhibitors Cell biology Kinetics Vascular endothelial growth factor A medicine.anatomical_structure Protein Biosynthesis Immunology medicine.drug |
| Zdroj: | Experimental Cell Research. 139:191-197 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(82)90332-9 |
| Popis: | To determine if human vascular endothelium influences the growth of smooth muscle cells (SMC), we have investigated the effect of media conditioned by cultured human vascular endothelial cells on smooth muscle cell proliferation. Cell growth was measured by [ 3 H]thymidine incorporation in endothelial and smooth muscle cells. The conditioned medium of confluent endothelial cells contains factors that inhibit the growth of actively dividing endothelial and smooth muscle cells. Media conditioned by proliferating endothelial cells or confluent smooth muscle cells were inactive. The generation of the inhibitory activity was time-dependent, not correlated with cell lysis and was demonstrated in both serum-free and serum-containing medium. Protein synthesis was involved in the generation of the inhibitory activity. Upon gel filtration, the inhibitory activity was associated with a substance having a molecular weight (MW) of >10 5 . Treatment with trypsin or alpha chymotrypsin did not abolish the activity. Furthermore, the activity remained stable after incubation for 30 min at 56 °C, whereas only 50% of the activity remained present after heating for 2 min at 100 °C. It is speculated that this inhibitory activity plays an important role in the regulation of smooth muscle cell proliferation. |
| Databáze: | OpenAIRE |
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