TNF‐α promoter gene polymorphisms in Spanish children with persistent oligoarticular and systemic‐onset juvenile idiopathic arthritis
| Autor: | Ana Patiño-García, J. Merino, J. García‐Consuegra, Luis Sierrasesúmaga, C Arnal, M. J. Rua, Elena Sotillo-Piñeiro, R. Merino, Consuelo Modesto |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Immunology
Arthritis Human leukocyte antigen Gene Frequency Rheumatology Polymorphism (computer science) Confidence Intervals Odds Ratio medicine Humans Immunology and Allergy Genetic Predisposition to Disease Child Promoter Regions Genetic Allele frequency Polymorphism Genetic Tumor Necrosis Factor-alpha business.industry Promoter General Medicine medicine.disease Arthritis Juvenile Systemic-onset juvenile idiopathic arthritis Spain Restriction fragment length polymorphism business Juvenile rheumatoid arthritis |
| Zdroj: | Scandinavian Journal of Rheumatology. 34:451-454 |
| ISSN: | 1502-7732 0300-9742 |
| DOI: | 10.1080/03009740510026652 |
| Popis: | To explore the possible association/s of the first reported tumour necrosis factor (TNF-alphaTNF-) alpha promoter gene polymorphisms -308, -238, -376 and -163 (G--A) with systemic (SoJIA) and oligoarticular subtypes of juvenile idiopathic arthritis (JIA); and to test the association between these polymorphisms and the class I/class II HLA alleles in our population.The patient group comprised 29 oligoarticular and 26 systemic Caucasian Spanish children with JIA; 68 healthy volunteers from the same ethnic group and geographical region served as controls. HLA alleles were determined using low-resolution polymerase chain reaction (PCR). TNF-alpha promoter gene polymorphisms were screened using PCR denaturing gradient gel electrophoresis (PCR-DGGE), followed, if positive, by restriction fragment length polymorphism (RFLP) analysis for identification.No statistical association was found between the four polymorphisms studied and JIA. However, the -308 G--A polymorphism (TNF A2) tended to be more frequent in patients with SoJIA than in the oligoarticular group. TNF A2 was strongly associated with the extended haplotype A1B8DR3 (p = 0.003), and the tandem polymorphism -238/-376 in the presence of B18 and DR3.The TNF A2 allele was more frequent in SoJIA than in the oligoarticular group. TNF A2 can help to create a more inflammatory milieu in this JIA subtype, in combination with other polymorphisms involved in regulatory sequences of key molecules in the inflammatory response. The association of the -308 and -238/-376 polymorphisms with specific alleles of the HLA is reconfirmed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |