Oxalate/hydrogen peroxide chemiluminescence reaction. A19F NMR probe of the reaction mechanism
| Autor: | Michael Barbush, Richard S. Givens, Ted Kuwana, Robert G. Carlson, Hitesh P. Chokshi, Richard L. Schowen |
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| Rok vydání: | 1990 |
| Předmět: |
Reaction mechanism
Magnetic Resonance Spectroscopy Clinical Biochemistry Reactive intermediate Inorganic chemistry Fluorine-19 NMR Photochemistry Biochemistry Peroxyoxalate Oxalate Analytical Chemistry law.invention chemistry.chemical_compound law Drug Discovery Hydrogen peroxide Molecular Biology Chemiluminescence Pharmacology Oxalates Aqueous solution Chemistry Fluorine Hydrogen Peroxide General Medicine Luminescent Measurements |
| Zdroj: | Biomedical Chromatography. 4:96-99 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.1130040304 |
| Popis: | The mechanism of the oxalate/hydrogen peroxide chemiluminescence reaction has been examined by magnetic resonance techniques. Investigation of the reactive intermediates involved in chemiluminescence was carried out with bis(2,6-difluorophenyl)oxalate (DFPO) using 19F NMR to probe its reactions with aqueous hydrogen peroxide. Formation and reactions of the intermediate hydroperoxy oxalate ester B along with the formation of the half ester product C and difluorophenol D were monitored by 19F NMR. When the reaction of DFPO and aqueous hydrogen peroxide was carried out in the presence of dansylphenylalanine, a typical fluorescent analyte, the intensity of the resonance due to the intermediate B was diminished in direct proportion to the concentration of the analyte. Comparison of the time/intensity profile of the chemiluminescence emission with that of the 19F NMR transient suggests that the hydroperoxy oxalate ester B is the likely 'reactive' intermediate, capable of participating in a chemically initiated electron exchange luminescence mechanism. |
| Databáze: | OpenAIRE |
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