Solid phase microextraction-comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry for the analysis of honey volatiles
| Autor: | Eva Klimánková, Tomas Cajka, Jana Hajslova, K. Holadová, Jack Cochran |
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| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Chromatography Time Factors Resolution (mass spectrometry) Analytical chemistry Temperature Filtration and Separation Honey Solid-phase microextraction Mass spectrometry Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry Two-dimensional chromatography Volatile organic compound Sample preparation Gas chromatography Time-of-flight mass spectrometry Volatilization Solid Phase Microextraction |
| Zdroj: | Journal of separation science. 30(4) |
| ISSN: | 1615-9306 |
| Popis: | Head-space solid phase microextration (SPME), followed by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS), has been implemented for the analysis of honey volatiles, with emphasis on the optimal selection of SPME fibre and the first- and second-dimension GC capillaries. From seven SPME fibres investigated, a divinylbenzene/Carboxen/polydimethylsiloxane (DVB/CAR/PDMS) 50/30 microm fibre provided the best sorption capacity and the broadest range of volatiles extracted from the headspace of a mixed honey sample. A combination of DB-5ms x SUPELCOWAX 10 columns enabled the best resolution of sample components compared to the other two tested column configurations. Employing this powerful analytical strategy led to the identification of 164 volatile compounds present in a honey mixture during a 19-min GC run. Combination of this simple and inexpensive SPME-based sampling/concentration technique with the advanced separation/identification approach represented by GCxGC-TOFMS allows a rapid and comprehensive examination of the honey volatiles profile. In this way, the laboratory sample throughput can be increased significantly and, at the same time, the risk of erroneous identification, which cannot be avoided in one-dimensional GC separation, is minimised. |
| Databáze: | OpenAIRE |
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