Differential Regulation and Function of 5′-Untranslated GR-Exon 1 Transcripts
| Autor: | Ian M. Adcock, Yvonne Bockmühl, Dietmar Spengler, Arleta Kuczynska, Osborne F. X. Almeida, Chris Murgatroyd |
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| Rok vydání: | 2011 |
| Předmět: |
Male
RNA Stability Thymus Gland Biology Kidney Cell Line Mice Exon Receptors Glucocorticoid Endocrinology Transcription (biology) Adrenal Glands Gene expression Protein biosynthesis Animals Humans RNA Messenger Promoter Regions Genetic Molecular Biology Gene Original Research Genetics Regulation of gene expression Base Sequence Alternative splicing Brain Promoter Exons General Medicine Rats Gene Expression Regulation Liver Organ Specificity Protein Biosynthesis Nucleic Acid Conformation 5' Untranslated Regions Spleen Signal Transduction |
| Zdroj: | Molecular Endocrinology. 25:1100-1110 |
| ISSN: | 1944-9917 0888-8809 |
| DOI: | 10.1210/me.2010-0436 |
| Popis: | Alternative splicing serves to increase biological diversity and adaptation. Many genes, including the glucocorticoid receptor (GR), contain multiple 5'-untranslated exons in their promoter regions that can give rise to various mRNA isoforms encoding the same protein. To date, information on the mouse GR promoter remains sparse. Here, we extensively characterize alternative first exons of the mouse GR to reveal homology to the rat and human. We further find that, although most promoters are broadly expressed in various tissues, transcription of individual promoters can be differentially regulated by growth factor- and depolarization-induced signaling. Moreover, in addition to selective promoter usage, the alternative first exon transcripts differentially control RNA stability and translation efficiency, indicative of their role in GR expression. In conclusion, the composite GR promoter enables multilayered adjustments in gene expression through transcriptional and posttranscriptional mechanisms that may serve varying physiological demands. |
| Databáze: | OpenAIRE |
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