Homocysteine reduces cholinesterase activity in rat and human serum
| Autor: | Cristiane Bastos de Mattos, Francieli M. Stefanello, Angela T. S. Wyse, Emilene B. S. Scherer, Carlos Alexandre Netto |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Serum medicine.medical_specialty Hyperhomocysteinemia Homocysteine medicine.medical_treatment Metabolite Homocystinuria chemistry.chemical_compound Subcutaneous injection Developmental Neuroscience In vivo Internal medicine medicine Animals Cholinesterases Humans Rats Wistar Saline Cholinesterase Analysis of Variance Dose-Response Relationship Drug biology business.industry Age Factors Middle Aged medicine.disease Rats Endocrinology Animals Newborn chemistry biology.protein Female business Developmental Biology |
| Zdroj: | International Journal of Developmental Neuroscience. 25:201-205 |
| ISSN: | 1873-474X 0736-5748 |
| DOI: | 10.1016/j.ijdevneu.2007.03.007 |
| Popis: | In the present study we investigated the effect of homocysteine administration, the main metabolite accumulating in homocystinuria, on cholinesterase activity in rat and human serum. For the in vivo study, 8-, 15- and 60-day-old rats received one subcutaneous injection of homocysteine (0.3, 0.4 or 0.6 μmol/g of body weight, respectively) or saline (control) and were sacrificed 1 h later, when serum was collected in order to determine cholinesterase activity. For the in vitro studies, serum of 8-, 15- and 60-day-old untreated rats or 20–25- and 52–60-day-old human beings (healthy volunteers) were incubated with 10–500 μM homocysteine. Results showed that acute hyperhomocysteinemia ( in vivo study) significantly reduced cholinesterase activity in the serum of rats of all ages tested. We also observed that 500 μM homocysteine added to the incubation medium ( in vitro study) significantly inhibited cholinesterase activity both in serum of rats and humans. Our findings seem to reinforce the proposed associations of cholinesterase activity with hyperhomocysteinemia. |
| Databáze: | OpenAIRE |
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