| Autor: |
Barth, Melanie, Bacioglu, Mehtap, Schwarz, Niklas, Novotny, Renata, Brandes, Janine, Welzer, Marc, Mazzitelli, Sonia, Häsler, Lisa M., Schweighauser, Manuel, Wuttke, Thomas V., Kronenberg-Versteeg, Deborah, Fog, Karina, Ambjørn, Malene, Alik, Ania, Melki, Ronald, Kahle, Philipp J., Shimshek, Derya R., Koch, Henner, Jucker, Mathias, Tanriöver, Gaye |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.15153929 |
| Popis: |
Additional file 1: Supplementary Fig. 1. αS pff characterization. (A, B) Proteolytic profiles of monomeric (A) and fibrillar (B) αS (100 M in PBS) after proteinase K treatment (3.7 μg/μl) at 37 °C. Aliquots were removed from the reaction at the time indicated (in min), immediately denatured with Laemmli buffer at 90 °C for 5 min and analysed on 15% PAGE. The gels were stained with Coomassie blue. The molecular weight markers are shown on the left of each gel. The αS fibrils reveal the typical proteolytic pattern as reported previously [30, 31]. (C, D) Transmission electron micrographs of the αS fibrils before (C) and after fragmentation (D). The scale bar represents 200 nm. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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