Comparison of ochratoxin A levels in edible pig tissues and in biological fluids after exposure to a contaminated diet
| Autor: | Giampiero Scortichini, Dragan Kovačević, Ivana Kmetič, Salvatore Milone, Nina Kudumija, Jelka Pleadin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Muscle tissue Ochratoxin A Pathology medicine.medical_specialty Swine 030106 microbiology Adipose tissue Enzyme-Linked Immunosorbent Assay Food Contamination Urine ANIMAL EXPOSURE Biology Toxicology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Animal science contaminated feed pigs tissues biological fluids medicine Biological fluids Animals Fluorometry Chromatography High Pressure Liquid 010401 analytical chemistry Animal Structures Contamination Ochratoxins Body Fluids Diet 0104 chemical sciences medicine.anatomical_structure Immunoassay method chemistry Biotechnology |
| Zdroj: | Mycotoxin Research. 32:145-151 |
| ISSN: | 1867-1632 0178-7888 |
| DOI: | 10.1007/s12550-016-0249-7 |
| Popis: | The aim of this study was to compare ochratoxin A (OTA) levels in pig tissues and biological fluids after animal exposure to contaminated diet (250 μg OTA/kg of feed) during 4 weeks of fattening. OTA concentrations were quantified using a validated immunoassay method (ELISA) and high-performance liquid chromatography with fluorescence detector (HPLC-FD). The highest mean OTA concentration in pig tissues was determined in kidneys of exposed animals (13.87 ± 1.41 μg/kg), followed by lungs (10.47 ± 1.97 μg/kg), liver (7.28 ± 1.75 μg/kg), spleen (4.81 ± 0.99 μg/kg), muscle tissue (4.72 ± 0.86 μg/kg), fat tissue (4.11 ± 0.88 μg/kg), heart (3.71 ± 1.09 μg/kg), and brain (3.01 ± 0.25 μg/kg). Furthermore, on the last day of exposure (day 28), significantly higher mean OTA levels were determined in urine (16.06 ± 3.09 μg/L) in comparison to serum (4.77 ± 1.57 μg/L) showing that OTA urine analysis could be a good marker to identify elevated levels of this contaminant in porcine tissues used for human consumption. This study gave guidelines for the most efficient OTA control in pig-derived biological materials that can be exercised at slaughterhouses. |
| Databáze: | OpenAIRE |
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