Comparison of an antigen capture enzyme-linked assay with reverse transcription - polymerase chain reaction and cell culture immunoperoxidase tests for the diagnosis of ruminant pestivirus infections
| Autor: | Sue Rowe, Jacqui Ralston, Kok-Mun Tham, Tania Houghton, Gary William Horner, Della Orr |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Enzyme-Linked Immunosorbent Assay Buffy coat Biology Polymerase Chain Reaction Sensitivity and Specificity Microbiology Virus law.invention Immunoenzyme Techniques Flaviviridae law Animals Polymerase chain reaction Diarrhea Viruses Bovine Viral Base Sequence General Veterinary Immunoperoxidase Pestivirus RNA-Directed DNA Polymerase General Medicine biology.organism_classification Virology Reverse transcription polymerase chain reaction Bovine Virus Diarrhea-Mucosal Disease Cattle Viral disease |
| Zdroj: | Veterinary Microbiology. 43:75-84 |
| ISSN: | 0378-1135 |
| Popis: | A study to compare the merits of three different tests for the diagnosis of ruminant pestivirus infections was carried out. Sensitivity studies using reference strains of bovine viral diarrhoea virus (BVDV) and buffy coat samples from persistently infected (PI) carriers showed the reverse transcription-polymerase chain reaction (RT-PCR) had a greater sensitivity than the other tests. The antigen capture enzyme-linked immunosorbent assay (ELISA) was least sensitive and could only be used on samples containing cells (tissue or blood). When 169 clinical samples were examined, the RT-PCR detected the most positives (42) compared to the ELISA (32) and the immunoperoxidase test (IPT) (20). The RT-PCR was more successful when specific antibody was also present in the sample. The lower sensitivity of the IPT was related to the use of a 1 passage (4-day) test and the testing of toxic or contaminated samples. The ELISA was found to be most suitable for large-scale testing for the diagnosis and control of pestivirus infections. |
| Databáze: | OpenAIRE |
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