A feedback loop between CaWRKY41 and H2O2 coordinates the response to Ralstonia solanacearum and excess cadmium in pepper
| Autor: | Shao Jian Zheng, Shuilin He, Gui Xin Li, Jinhui Lin, Yongping Chen, Deyi Guan, Fengfeng Dang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Capsicum annuum Physiology cadmium H2O2 Mutant Arabidopsis Heterologous Plant Science 01 natural sciences Microbiology 03 medical and health sciences Pepper Plant Immunity Transcription factor Disease Resistance Plant Diseases Plant Proteins reactive oxygen species Ralstonia solanacearum biology Chemistry fungi food and beverages Hydrogen Peroxide biochemical phenomena metabolism and nutrition biology.organism_classification Research Papers WRKY protein domain 030104 developmental biology Plant—Environment Interactions CaWRKY41 biology.protein Capsicum 010606 plant biology & botany Peroxidase Transcription Factors |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | CaWRKY41 plays dual roles in increasing resistance to Ralstonia solanacearum infection and susceptibility to Cd stress in pepper by indirectly and directly increasing H2O2 accumulation and Zn transporter expression. WRKY transcription factors have been implicated in both plant immunity and plant responses to cadmium (Cd); however, the mechanism underlying the crosstalk between these processes is unclear. Here, we characterized the roles of CaWRKY41, a group III WRKY transcription factor, in immunity against the pathogenic bacterium Ralstonia solanacearum and Cd stress responses in pepper (Capsicum annuum). CaWRKY41 was transcriptionally up-regulated in response to Cd exposure, R. solanacearum inoculation, and H2O2 treatment. Virus-induced silencing of CaWRKY41 increased Cd tolerance and R. solanacearum susceptibility, while heterologous overexpression of CaWRKY41 in Arabidopsis impaired Cd tolerance, and enhanced Cd and zinc (Zn) uptake and H2O2 accumulation. Genes encoding reactive oxygen species-scavenging enzymes were down-regulated in CaWRKY41-overexpressing Arabidopsis plants, whereas genes encoding Zn transporters and enzymes involved in H2O2 production were up-regulated. Consistent with these findings, the ocp3 (overexpressor of cationic peroxidase 3) mutant, which has elevated H2O2 levels, displayed enhanced sensitivity to Cd stress. These results suggest that a positive feedback loop between H2O2 accumulation and CaWRKY41 up-regulation coordinates the responses of pepper to R. solanacearum inoculation and Cd exposure. This mechanism might reduce Cd tolerance by increasing Cd uptake via Zn transporters, while enhancing resistance to R. solanacearum. |
| Databáze: | OpenAIRE |
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