Ica-expression and gentamicin susceptibility of Staphylococcus epidermidis biofilm on orthopedic implant biomaterials

Autor: Henny C. van der Mei, Abu Tholib Aman, Henk J. Busscher, Titik Nuryastuti, Bastiaan P. Krom
Přispěvatelé: Preventive Dentistry, Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT)
Rok vydání: 2010
Předmět:
Micrococcaceae
Antibiotics
Biocompatible Materials
medicine.disease_cause
biofilm
CULTURE
Prostheses and Implants/microbiology
Biofilms/drug effects
gentamicin-susceptibility
Staphylococcus epidermidis
OPERON
Microscopy
ICAADBC
Microscopy
Confocal
biology
Bacterial/drug effects
Metals and Alloys
Biomaterial
Prostheses and Implants
Gentamicins/pharmacology
INFECTIONS
Confocal
BACTERIA
orthopedics
Gentamicin
Staphylococcus epidermidis/cytology
Biocompatible Materials/pharmacology
medicine.drug
Materials science
Prosthesis-Related Infections
medicine.drug_class
Biomedical Engineering
Microbial Sensitivity Tests
Microbiology
Arthroplasty
Biomaterials
Bacterial Proteins
ica-expression
medicine
AUREUS
PIA
Gene Expression Regulation
Bacterial/drug effects
Prosthesis-Related Infections/microbiology
Pseudomonas aeruginosa
Bacterial Proteins/genetics
PSEUDOMONAS-AERUGINOSA
Biofilm
Gene Expression Regulation
Bacterial
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
infection
S. epidermidis
Gene Expression Regulation
Biofilms
POLYSACCHARIDE INTERCELLULAR ADHESIN
Ceramics and Composites
Gentamicins
SKIN
Bacteria
Zdroj: Nuryastuti, T, Krom, B P, Aman, A T, Busscher, H J & van der Mei, H C 2011, ' Ica-expression and gentamicin susceptibility of Staphylococcus epidermidis biofilm on orthopedic implant biomaterials ', Journal of Biomedical Materials Research. Part A, vol. 96, no. 2, pp. 365-71 . https://doi.org/10.1002/jbm.a.32984
Journal of Biomedical Materials Research. Part A, 96(2), 365-71. John Wiley and Sons Inc.
Journal of Biomedical Materials Research. Part A, 96A(2), 365-371. Wiley
ISSN: 1552-4965
1549-3296
DOI: 10.1002/jbm.a.32984
Popis: Ica-expression by Staphylococcus epidermidis and slime production depends on environmental conditions such as implant material and presence of antibiotics. Here, we evaluate biofilm formation and ica-expression of S. epidermidis strains on biomaterials involved in total hip-and knee arthroplasty [polyethylene (PE), polymethylmethacrylate (PMMA), stainless steel (SS)]. Ica-expression, assayed using real-time RT-PCR, was highest on PE as confirmed using confocal laser scanning microscopy. Yet biofilm formation by S. epidermidis was most extensive on SS, with less slime production. Ica-expression and slime production were minimal on PMMA. After 3 h of continued growth of 24 h old biofilms in the presence of gentamicin, biofilms on PE showed lower susceptibility to gentamicin, relative to the other materials, presumably as a result of the stronger ica-expression. A higher gentamicin concentration further decreased metabolic activity on all biomaterials. It is concluded that the level of biomaterial-induced ica-expression does not correlate with the amount of biofilm formed, but initially aids bacteria in surviving antibiotic attacks. Once antibiotic treatment has started however, also the antibiotic itself induces slime production and only if its concentration is high enough, killing results. Results suggest that biomaterial-associated infections in orthopedics by S. epidermidis on PE may be more difficult to eradicate than on PMMA or SS. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 365-371, 2011.
Databáze: OpenAIRE
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