Drop-delay measurement using enzyme-coated particles
| Autor: | Andrey I. Poletaev, V. V. Zenin, Yuri Lazebnik |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Time Factors
Materials science Ranidae Particle number Maximum level Drop (liquid) Biophysics Cell Separation Cell Biology Hematology Flow Cytometry Blood Cell Count Pathology and Forensic Medicine Flow sorting Endocrinology Microscopy Fluorescence Nephelometry and Turbidimetry Animals sort Benzimidazoles Fluorescein-5-isothiocyanate Horseradish Peroxidase Biomedical engineering |
| Zdroj: | Cytometry. 13:649-652 |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/cyto.990130614 |
| Popis: | A simple technique is suggested for the measurement of drop delay for flow sorting. While the flow cytometer was set to sort a fixed number of particles, the drop-delay setting was changed step by step, and at each step the HRP-coupled particles were sorted into a well of an immunoassay strip. Then the HRP activity of the sorted samples was revealed by routine methods. The maximum level of the enzyme activity shows the proper drop-delay setting. Determination of the drop-delay setting takes only a few minutes. The technique is independent of the type of flow cytometer and does not require any additional equipment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text