Exogenous cortisol and red light irradiation affect reproductive parameters in the goldfish Carassius auratus
| Autor: | Young-Su Park, Cheol Young Choi, Heung-Sik Park, Kang Hee Kho, Jin Ah Song |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
medicine.medical_specialty Hydrocortisone Light Physiology media_common.quotation_subject Aquatic Science Biology Biochemistry Gonadotropin-Releasing Hormone Internal medicine Goldfish medicine Carassius auratus Animals Testosterone Red light RNA Messenger media_common Estradiol Reproduction General Medicine Endocrinology Mrna level Hormone receptor Follicle Stimulating Hormone beta Subunit Double immunofluorescence staining hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Fish physiology and biochemistry. 47(6) |
| ISSN: | 1573-5168 |
| Popis: | Reproductive hormones play essential roles in the control of reproduction and gonadal maturation in fish. The purpose of this study was to determine the effects of cortisol administration (10 µg/g or 50 µg/g) or red light irradiation at two intensities (0.5 W/m2 or 1.0 W/m2) on the reproductive hormones in goldfish (Carassius auratus). The effects of different treatments were analyzed by determining the mRNA expression levels of gonadotropin-inhibitory hormone receptor (GnIH-R), chicken gonadotropin-releasing hormone (cGnRH-II), salmon GnRH (sGnRH), FSHβ, LHβ, and plasma testosterone and the level of 17β-estradiol for 48 h. Additionally, by double immunofluorescence staining, we detected the expression of both GnIH and GnRH in the diencephalons of goldfish brains. The mRNA expression of GnIH-R was significantly higher in the cortisol group and red light-irradiated group from 3 to 48 h than in the control group. Additionally, the mRNA levels of cGnRH-II, sGnRH, FSHβ, LHβ, testosterone, and 17β-estradiol were significantly lower in the cortisol group than in the other groups from 3 to 48 h. These results indicated that both cortisol and red light-emitting diode (LED) light increased GnIH expression and inhibited GnRH expression. In particular, red light irradiation suppressed reproductive responses as much as the cortisol treatment at 48 h. Thus, it could be an alternative method for suppressing reproductive responses in future aquacultures. |
| Databáze: | OpenAIRE |
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