Novel Propagation Strategy of Saccharomyces cerevisiae for Enhanced Xylose Metabolism during Fermentation on Softwood Hydrolysate
| Autor: | Henrique César Teixeira Veras, Andreea Cristina Dobrescu, Cristiano Varrone, Jan Dines Knudsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Sucrose
Fermentation industries. Beverages. Alcohol Saccharomyces cerevisiae Plant Science Xylose non-fermentable carbon sources Biochemistry Genetics and Molecular Biology (miscellaneous) Hydrolysate automated propagation strategy chemistry.chemical_compound Xylose metabolism Glycerol second-generation bioethanol softwood hydrolysate Automated propagation strategy Non-fermentable carbon sources Second-generation bioethanol Food science C5/C6 co-consumption TP500-660 biology food and beverages biology.organism_classification Yeast chemistry Softwood hydrolysate xylose Fermentation Food Science |
| Zdroj: | Dobrescu, A-C, Veras, H C T, Varrone, C & Knudsen, J D 2021, ' Novel Propagation Strategy of Saccharomyces cerevisiae for Enhanced Xylose Metabolism during Fermentation on Softwood Hydrolysate ', Fermentation, vol. 7, no. 4, 288 . https://doi.org/10.3390/fermentation7040288 Fermentation; Volume 7; Issue 4; Pages: 288 Fermentation, Vol 7, Iss 288, p 288 (2021) |
| DOI: | 10.3390/fermentation7040288 |
| Popis: | An economically viable production of second-generation bioethanol by recombinant xylose-fermenting Saccharomyces cerevisiae requires higher xylose fermentation rates and improved glucose–xylose co-consumption. Moreover, xylose-fermenting S. cerevisiae recognises xylose as a non-fermentable rather than a fermentable carbon source, which might partly explain why xylose is not fermented into ethanol as efficiently as glucose. This study proposes propagating S. cerevisiae on non-fermentable carbon sources to enhance xylose metabolism during fermentation. When compared to yeast grown on sucrose, cells propagated on a mix of ethanol and glycerol in shake flasks showed up to 50% higher xylose utilisation rate (in a defined xylose medium) and a double maximum fermentation rate, together with an improved C5/C6 co-consumption (on an industrial softwood hydrolysate). Based on these results, an automated propagation protocol was developed, using a fed-batch approach and the respiratory quotient to guide the ethanol and glycerol-containing feed. This successfully produced 71.29 ± 0.91 g/L yeast with an average productivity of 1.03 ± 0.05 g/L/h. These empirical findings provide the basis for the design of a simple, yet effective yeast production strategy to be used in the second-generation bioethanol industry for increased fermentation efficiency. |
| Databáze: | OpenAIRE |
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