The Synchronization of Replication and Division Cycles in Individual E. coli Cells
| Autor: | Ebba G. Lundius, Johan Elf, David Fange, Özden Baltekin, Mats Wallden |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
DNA Replication DNA Bacterial Adder Cell division DNA replication Chromosome Biology Division (mathematics) Chromosomes Bacterial Models Biological General Biochemistry Genetics and Molecular Biology Synchronization Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Replication (statistics) Escherichia coli Growth rate 030217 neurology & neurosurgery Cell Division |
| Zdroj: | Cell. 166(3) |
| ISSN: | 1097-4172 |
| Popis: | Isogenic E. coli cells growing in a constant environment display significant variability in growth rates, division sizes, and generation times. The guiding principle appears to be that each cell, during one generation, adds a size increment that is uncorrelated to its birth size. Here, we investigate the mechanisms underlying this "adder" behavior by mapping the chromosome replication cycle to the division cycle of individual cells using fluorescence microscopy. We have found that initiation of chromosome replication is triggered at a fixed volume per chromosome independent of a cell's birth volume and growth rate. Each initiation event is coupled to a division event after a growth-rate-dependent time. We formalize our findings in a model showing that cell-to-cell variation in division timing and cell size is mainly driven by variations in growth rate. The model also explains why fast-growing cells display adder behavior and correctly predict deviations from the adder behavior at slow growth. |
| Databáze: | OpenAIRE |
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