Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
| Autor: | María de la Paz Santangelo, María Fiorella Alvarado Pinedo, Magali Andrea Romero, María Isabel Romano, Roberto Damián Moyano, Gabriel Eduardo Travería, Belén Rocío Imperiale |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Quantitative Trait Loci lcsh:QR1-502 Paratuberculosis Locus (genetics) Biology Genetic Isolation Variación Genética lcsh:Microbiology law.invention mycobacterium avium sp. paratuberculosis Tandem repeat law Genetic variation medicine Infección por Mycobacterium avium Polymerase chain reaction Genetics Loci de Rasgos Cuantitativos Genetic diversity Aislamiento Genético Genetic Variation automated mycobacterial interspersed repetitive unit–variable number tandem repeat typing genetic diversity medicine.disease multi-locus short-sequence repeat typing Mycobacterium avium subsp. paratuberculosis Variable number tandem repeat Infectious Diseases Mycobacterium avium Infections Genetic isolate |
| Zdroj: | International Journal of Mycobacteriology 10 (1) : 51-59 (Marzo 2021) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA International Journal of Mycobacteriology, Vol 10, Iss 1, Pp 51-59 (2021) |
| Popis: | Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern. Instituto de Biotecnología Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Imperiale, Belen Rocio. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX); Argentina Fil: Imperiale, Belen Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero, Magalí. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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