In vitro study of embryotoxic and dysmorphogenic effects of mercuric chloride and methylmercury chloride in the rat
| Autor: | Kirk T. Kitchin, M.T. Ebron, D. Svendsgaard |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
Toxicology Chloride chemistry.chemical_compound Organ Culture Techniques medicine Animals In vitro study Incubation Growth retardation Embryogenesis Rats Inbred Strains Mercury General Medicine Glutathione Methylmercury Compounds Embryo Mammalian Molecular biology Rats Teratogens chemistry Biochemistry Methylmercury chloride Mercuric Chloride Female Food Science medicine.drug Explant culture |
| Zdroj: | Food and Chemical Toxicology. 22:31-37 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/0278-6915(84)90049-8 |
| Popis: | Rat-embryo explants removed on day 10.5 of gestation were cultured for 48 hr in various concentrations of HgCl 2 , CH 3 HgCl or glutathione. Dose-related dysmorphogenesis and growth retardation occurred with increasing concentrations of HgCl 2 (1–10 μ m ). Increasing concentrations of CH 3 HgCl (3–100 μ m ) produced a similar pattern of embryonic effects. The rat-serum incubation medium had no detectable level of reduced glutathione ( m ) and only 0.12 m m total sulphydryl groups. Reduced glutathione (60–300 μ m ) added to the incubation medium was relatively non-toxic. The addition of exogenous glutathione to culture medium containing HgCl 2 partially antagonized the embryonic growth retardation and prevented most of the embryolethality observed in cultures to which only HgCl 2 had been added. |
| Databáze: | OpenAIRE |
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