The role of nitrogen off-stoichiometry in the osteogenic behavior of silicon nitride bioceramics
| Autor: | Francesco Boschetto, Satoshi Horiguchi, Alfredo Rondinella, Wenliang Zhu, Bryan J. McEntire, Matteo Zanocco, Ryan M. Bock, Elia Marin, Giuseppe Pezzotti, B. Sonny Bal |
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| Rok vydání: | 2019 |
| Předmět: |
Ceramics
Materials science Silicon Nitrogen chemistry.chemical_element Bioengineering 02 engineering and technology Cell Line Tumor Humans Osteogenesis Materials Testing Silicon Compounds 010402 general chemistry Bone tissue 01 natural sciences Cell Line Biomaterials chemistry.chemical_compound symbols.namesake X-ray photoelectron spectroscopy medicine Spectroscopy Tumor 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure Silicon nitride chemistry Chemical engineering Mechanics of Materials symbols Atomic ratio 0210 nano-technology Raman spectroscopy Stoichiometry |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 105 |
| ISSN: | 1873-0191 |
| Popis: | The surface chemistry of silicon nitride plays an important role in stimulating osteoblasts to proliferate and produce bone tissue with improved efficiency. This property, which is advantageous in spinal fusion surgery has a chemical origin and is a direct consequence of the cleavage of covalent SN bonds in an aqueous environment. Building upon a wealth of published research on the stimulation of osteoblastic activity by silicon, the aim of this paper is to explore the role of nitrogen and, more specifically, the N/Si atomic ratio on the osteogenic response of Si3N4. The surface stoichiometry of Si3N4 was gradually altered toward a silicon-rich composition by systematically treating the Si3N4 surface with a high-power pulsed laser in an Ar gas atmosphere (i.e., operated at different pulse times, spot sizes, and voltages). Different analytical probes were used to characterize the surface including X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and energy dispersive X-ray spectroscopy (EDS). Osteoconductivity was tested in vitro using SaOS-2 osteosarcoma cells, and samples with different surface stoichiometry were compared for their osteogenic response. These experiments clearly indicated a fundamental role for nitrogen off-stoichiometry in osteogenesis, and showed that both cell proliferation and growth of bone tissue diminished with decreasing nitrogen content. |
| Databáze: | OpenAIRE |
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