The Ihh signal is essential for regulating proliferation and hypertrophy of cultured chicken chondrocytes
| Autor: | R.S. Ma, Zhiguo Zhou, J.F. Hou, Hongsheng Zhang, J.W. Luo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
animal structures Indian hedgehog Cyclopamine Bone Morphogenetic Protein 6 Physiology Type II collagen Biology Biochemistry Chondrocyte Muscle hypertrophy chemistry.chemical_compound Chondrocytes Western blot Internal medicine medicine Animals Hedgehog Proteins Molecular Biology Cell Proliferation medicine.diagnostic_test Parathyroid hormone-related protein Parathyroid Hormone-Related Protein Gene Expression Regulation Developmental Cell Differentiation Hypertrophy biology.organism_classification Cell biology body regions Bone morphogenetic protein 6 medicine.anatomical_structure Endocrinology chemistry Collagen Chickens Signal Transduction |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 166:117-122 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/j.cbpb.2013.07.010 |
| Popis: | The Indian hedgehog (Ihh) signal plays a vital role in regulating proliferation and hypertrophy of chondrocytes. To investigate its function in postnatal chicken (Gallus gallus) chondrocytes, cyclopamine was used to inhibit Ihh signaling. The MTT and ALP assays revealed the downgrade-proliferation and upgrade-differentiation of chondrocytes. To further elucidate the mechanism, the mRNA expression levels of Ihh, parathyroid hormone related protein (PTHrP), Gli-2, Bcl-2, Bone Morphogenetic Protein 6 (BMP-6), type X collagen (Col X) and type II collagen (Col II) were detected by quantitative real-time RT-PCR analysis, and the protein expressions of Ihh, Col X, and Col II were determined using Western blot analysis. After the Ihh signal was blocked, chondrocytes demonstrated high expression levels of PTHrP and Col X and low levels of Gli-2, BMP-6, Bcl-2 and Col II although Ihh expression was increased. Based on these results, the Ihh signal is essential for balancing chicken chondrocyte proliferation and hypertrophy, and the regulatory function of PTHrP acts in an Ihh-dependent manner. Furthermore, BMP-6 and Bcl-2 played roles in maintaining the development of chondrocytes and may be downstream regulatory factors of Ihh signaling. |
| Databáze: | OpenAIRE |
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