Polymerase chain reaction protocols for the detection of DNA from mucosal human papillomavirus types -6, -11, -16, -18, -31 and -33
| Autor: | Jennifer M. Best, Barbara Kell, Christine Mant, John Cason |
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| Rok vydání: | 1997 |
| Předmět: |
Time Factors
Biology Malignancy Polymerase Chain Reaction Virus law.invention chemistry.chemical_compound law Virology medicine Humans Papillomaviridae Cervix Polymerase chain reaction Cervical cancer Mucous Membrane Papillomavirus Infections virus diseases medicine.disease Anus female genital diseases and pregnancy complications Tumor Virus Infections medicine.anatomical_structure Sexual abuse chemistry DNA Viral DNA |
| Zdroj: | Journal of Virological Methods. 66:169-178 |
| ISSN: | 0166-0934 |
| Popis: | Individual types of human papillomaviruses (HPV) which infect mucosal surfaces have been implicated as the causative agents for carcinomas of the cervix, anus, penis, larynx and the buccal cavity, occasional periungal carcinomas, as well a:s benign anogenital warts. The identification of particular HPV types is thus important for: identifying patients with premalignant lesions who are at risk of progression to malignancy; epidemiological studies; studies of the natural history of these viruses; and even medico-legal cases of suspected sexual abuse of children. In this protocol we describe PCR assays for: the identification of DNA from the mucosal HPVs types -6, -11, -16, -18, -31 and -33; a consensus HPV PCR for detecting DNA from 20 characterised mucosal HPVs, as well as more than 25 novel HPVs; and, for a control PCR for β-globin. |
| Databáze: | OpenAIRE |
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