Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans
| Autor: | Bao-Ling Li, Xing-Meng Lu, Guo-Jun Lang, Chuan-Xi Zhang, Linlin Yu, Ming-Yan Zhang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Insecticides
Aché viruses Molecular Sequence Data Mutant Biology Biochemistry Toxicology chemistry.chemical_compound Bombyx mori Complementary DNA Dichlorvos Animals Point Mutation Amino Acid Sequence Omethoate Molecular Biology Diptera fungi General Medicine Bombyx biology.organism_classification Acetylcholinesterase Molecular biology Recombinant Proteins language.human_language Amino Acid Substitution chemistry Chlorpyrifos language Cholinesterase Inhibitors Sequence Alignment |
| Zdroj: | BMB Reports. 43:573-578 |
| ISSN: | 1976-6696 |
| DOI: | 10.5483/bmbrep.2010.43.8.573 |
| Popis: | Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus- insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I(50) values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori. |
| Databáze: | OpenAIRE |
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