Extracts of Renealmia alpinia (Rottb.) MAAS Protect against Lethality and Systemic Hemorrhage Induced by Bothrops asper Venom: Insights from a Model with Extract Administration before Venom Injection
| Autor: | José María Gutiérrez, Arley Camilo Patiño, Alexandra Rucavado, Juan C Quintana, Jaime Andrés Pereañez, Dora Benjumea |
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| Rok vydání: | 2015 |
| Předmět: |
Snake venom
Health Toxicology and Mutagenesis Antidotes Renealmia alpinia lcsh:Medicine Snake Bites Hemorrhage Venom Pharmacology Kidney Toxicology complex mixtures Article Micropropagated plants law.invention Mice serine proteinase Zingiberaceae law Crotalid Venoms Animals Bothrops Lung micropropagated plants biology Plant Extracts Myocardium lcsh:R Lethal dose Alpinia biology.organism_classification Metalloproteinases Anti-venom activity snake venoms anti-venom activity metalloproteinase Phytotherapy Serine proteinase |
| Zdroj: | Toxins; Volumen 7, Número 5. 2015 Kérwá Universidad de Costa Rica instacron:UCR Toxins Toxins, Vol 7, Iss 5, Pp 1532-1543 (2015) Volume 7 Issue 5 Pages 1532-1543 |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins7051532 |
| Popis: | Renealmia alpinia (Rottb.) MAAS, obtained by micropropagation (in vitro) and wild forms have previously been shown to inhibit some toxic activities of Bothrops asper snake venom if preincubated before injection. In this study, assays were performed in a murine model in which extracts were administered for three days before venom injection. R. alpinia extracts inhibited lethal activity of B. asper venom injected by intraperitoneal route. Median Effective Dose (ED50) values were 36.6 ± 3.2 mg/kg and 31.7 ± 5.4 mg/kg (p > 0.05) for R. alpinia wild and in vitro extracts, respectively. At a dose of 75 mg/kg, both extracts totally inhibited the lethal activity of the venom. Moreover, this dose prolonged survival time of mice receiving a lethal dose of venom by the intravenous route. At 75 mg/kg, both extracts of R. alpinia reduced the extent of venom-induced pulmonary hemorrhage by 48.0% (in vitro extract) and 34.7% (wild extract), in agreement with histological observations of lung tissue. R. alpinia extracts also inhibited hemorrhage in heart and kidneys, as evidenced by a decrease in mg of hemoglobin/g of organ. These results suggest the possibility of using R. alpinia as a prophylactic agent in snakebite, a hypothesis that needs to be further explored. |
| Databáze: | OpenAIRE |
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