Non-cytotoxic Concentration of Cisplatin Decreases Neuroplasticity-Related Proteins and Neurite Outgrowth Without Affecting the Expression of NGF in PC12 Cells
| Autor: | Rafaela Scalco Ferreira, Laís Silva Fernandes, Antonio Cardozo dos Santos, Neife Aparecida Guinaim dos Santos, Nádia Maria Martins |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Synapsin I Neurite Cellular differentiation Neuronal Outgrowth Down-Regulation Receptors Nerve Growth Factor Tropomyosin receptor kinase A Biochemistry PC12 Cells 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine GAP-43 Protein Nerve Growth Factor medicine Neurites Animals Gap-43 protein Cisplatin Neuronal Plasticity biology Cell Differentiation General Medicine Axons Cell biology Rats 030104 developmental biology Nerve growth factor nervous system biology.protein Synaptophysin 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neurochemical research. 41(11) |
| ISSN: | 1573-6903 |
| Popis: | Cisplatin is the most effective and neurotoxic platinum chemotherapeutic agent. It induces a peripheral neuropathy characterized by distal axonal degeneration that might progress to degeneration of cell bodies and apoptosis. Most symptoms occur nearby distal axonal branches and axonal degeneration might induce peripheral neuropathy regardless neuronal apoptosis. The toxic mechanism of cisplatin has been mainly associated with DNA damage, but cisplatin might also affect neurite outgrowth. Nevertheless, the neurotoxic mechanism of cisplatin remains unclear. We investigated the early effects of cisplatin on axonal plasticity by using non-cytotoxic concentrations of cisplatin and PC12 cells as a model of neurite outgrowth and differentiation. PC12 cells express NGF-receptors (trkA) and respond to NGF by forming neurites, branches and synaptic vesicles. For comparison, we used a neuronal model (SH-SY5Y cells) that does not express trkA nor responds to NGF. Cisplatin did not change NGF expression in PC12 cells and decreased neurite outgrowth in both models, suggesting a NGF/trkA independent mechanism. It also reduced axonal growth (GAP-43) and synaptic (synapsin I and synaptophysin) proteins in PC12 cells, without inducing mitochondrial damage or apoptosis. Therefore, cisplatin might affect axonal plasticity before DNA damage, NGF/trkA down-regulation, mitochondrial damage or neuronal apoptosis. This is the first study to show that neuroplasticity-related proteins might be early targets of the neurotoxic action of cisplatin and their role on cisplatin-induced peripheral neuropathy should be investigated in vivo. |
| Databáze: | OpenAIRE |
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