Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction
| Autor: | Mahmoud Elhariri, Mona A. El-Shabrawy, Gad El-Said Wagih, E. A. Elgabry, Sabry D Morgan, Azza S. M. Abuelnaga, Asmaa Samy Mansour |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine Enterotoxin Biology medicine.disease_cause SF1-1100 Microbiology 03 medical and health sciences Multiplex polymerase chain reaction SF600-1100 medicine Bulk tank General Veterinary Toxin raw milk food and beverages Raw milk S. aureus Latex fixation test Staphylococcal Food Poisoning Animal culture 030104 developmental biology Staphylococcus aureus reversed passive latex agglutination enterotoxin genes multiplex polymerase chain reaction Research Article |
| Zdroj: | Veterinary World Veterinary World, Vol 10, Iss 8, Pp 843-847 (2017) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Aim This review gives an outline of the assessment of enterotoxigenic Staphylococcus aureus tainting levels in raw milk from different sources in Egypt and characterization of enterotoxigenic strains utilizing a technique in light of PCR to identify genes coding for the production of staphylococcal enterotoxin (SE). The obtained data were compared with results from the application of the reversed passive latex. Materials and methods Multiplex PCR and reversed passive latex agglutination (RPLA) were used. A total of 141 samples of raw milk (cow's milk=33, buffalo's milk=58, and bulk tank milk=50) were investigated for S. aureus contamination and tested for enterotoxin genes presence and toxin production. Results S. aureus was detected in 23 (16.3%) samples phenotypically and genotypically by amplification of nuc gene. The S. aureus isolates were investigated for SEs genes (sea to see) by multiplex PCR and the toxin production by these isolates was screened by RPLA. SEs genes were detected in six isolates (26.1%) molecularly; see was the most observed gene where detected in all isolates, two isolates harbored seb, and two isolates harbored sec. According to RPLA, three isolates produced SEB and SEC. Conclusion The study revealed the widespread of S. aureus strains caring genes coding for toxins. The real significance of the presence of these strains or its toxins in raw milk and their possible impact a potential hazard for staphylococcal food poisoning by raw milk consumption. Therefore, detection of enterotoxigenic S. aureus strains in raw milk is necessary for consumer safety. |
| Databáze: | OpenAIRE |
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