Restricted Immunoglobulin Variable Region (Ig V) Gene Expression Accompanies Secondary Rearrangements of Light Chain Ig V Genes in Mouse Plasmacytomas
| Autor: | Gary M. Jones, D. Siwarski, Guillaume Dighiero, Allen E. Coleman, Jennifer Kim, Lena Diaw, Konrad Huppi |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Idiotype
Immunology Population Molecular Sequence Data Immunoglobulin Variable Region Biology Immunoglobulin light chain Mice plasmacytoma V gene usage immune system diseases allelic exclusion medicine Immunology and Allergy Animals Amino Acid Sequence education Gene Gene Rearrangement Homeodomain Proteins education.field_of_study Mice Inbred BALB C polyreactivity Base Sequence Genes Immunoglobulin Reverse Transcriptase Polymerase Chain Reaction Gene rearrangement medicine.disease Molecular biology DNA-Binding Proteins Allelic exclusion V(D)J rearrangement Mice Inbred DBA biology.protein Plasmacytoma Original Article Immunoglobulin Light Chains Antibody |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | The many binding studies of monoclonal immunoglobulin (Ig) produced by plasmacytomas have found no universally common binding properties, but instead, groups of plasmacytomas with specific antigen-binding activities to haptens such as phosphorylcholine, dextrans, fructofuranans, or dinitrophenyl. Subsequently, it was found that plasmacytomas with similar binding chain specificities not only expressed the same idiotype, but rearranged the same light (VL) and heavy (VH) variable region genes to express a characteristic monoclonal antibody. In this study, we have examined by enzyme-linked immunosorbent assay five antibodies secreted by silicone-induced mouse plasmacytomas using a broader panel of antigens including actin, myosin, tubulin, single-stranded DNA, and double-stranded DNA. We have determined the Ig heavy and light chain V gene usage in these same plasmacytomas at the DNA and RNA level. Our studies reveal: (a) antibodies secreted by plasmacytomas bind to different antigens in a manner similar to that observed for natural autoantibodies; (b) the expressed Ig heavy genes are restricted in V gene usage to the VH-J558 family; and (c) secondary rearrangements occur at the light chain level with at least three plasmacytomas expressing both κ and λ light chain genes. These results suggest that plasmacytomas use a restricted population of B cells that may still be undergoing rearrangement, thereby bypassing the allelic exclusion normally associated with expression of antibody genes. |
| Databáze: | OpenAIRE |
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