Degradation of water-insoluble dyes by microperoxidase-11, an effective and stable peroxidative catalyst in hydrophilic organic media
| Autor: | Mari Kabuto, Masafumi Oyadomari, Hiroyuki Wariishi, Junko Mikuni, Hiroo Tanaka |
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| Rok vydání: | 2002 |
| Předmět: |
Industrial Waste
Cytochrome c Group Pyrogallol Anthraquinone Catalysis Reaction rate chemistry.chemical_compound Oxidizing agent Organic chemistry Animals Horses Solubility Coloring Agents Substrate (chemistry) Biodegradation Hydrogen-Ion Concentration Kinetics Biodegradation Environmental chemistry Peroxidases Solvents Environmental Pollutants Methanol Azo Compounds Biotechnology |
| Zdroj: | Biotechnology progress. 18(1) |
| ISSN: | 8756-7938 |
| Popis: | Microperoxidase-11 (MP-11), a heme-containing undecapeptide, derived from horse heart cytochrome c was utilized as a peroxidative catalyst. Catalytic characteristics of MP-11 in hydrophilic organic media were studied using 2,6-dimethoxyphenol as a reducing substrate in a series of organic solvents at various concentrations, indicating that MP-11 was active in water-miscible organic solvents but at least 5% water was compulsory for the catalytic action. Thus, MP-11 was not active in hydrophobic solvents. The pH of the water portion in the media affected the reaction rate. The optimal pH was found to be 9, where a release of protons from either an oxidizing or reducing substrate to the media was facilitated. The decolorization of water-insoluble synthetic dyes by MP-11 in 90% methanol was attempted. MP-11 showed effective decolorization activities against either azo or anthraquinone dyes. The degradation pathway for Solvent Orange 7 was investigated in detail, showing that MP-11 catalyzed the oxidative cleavage of the azo linkage to generate 1,2-naphthoquinone and 2,4-dimethylphenol as key intermediates. |
| Databáze: | OpenAIRE |
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