Determination of eight phosphatidylethanol homologues in blood by reversed phase liquid chromatography–tandem mass spectrometry – How to avoid co-elution of phosphatidylethanols and unwanted phospholipids

Autor:	Marisa Maria, Benedicte Jørgenrud, Thomas Berg
Rok vydání:	2022
Předmět:	Chromatography Reverse-Phase Alcohol Drinking Ethanol Organic Chemistry Glycerophospholipids General Medicine Biochemistry Analytical Chemistry Tandem Mass Spectrometry Solvents Phospholipids Biomarkers Chromatography High Pressure Liquid Chromatography Liquid
Zdroj:	Journal of Chromatography A. 1684:463566
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2022.463566
Popis:	Phosphatidylethanols (PEths) are specific, direct alcohol biomarkers with a substantially longer half-life than ethanol, and can be used to distinguish between heavy- and social drinking. More than forty PEth homologues have been detected in blood from heavy drinkers, and PEth 16:0/18:1 is the predominant one. Since PEths are phospholipids it can be difficult to isolate them from unwanted phospholipids during sample preparation. To minimize possible matrix effects it is therefore important to separate PEths from other phospholipids during LC-MS/MS analysis. In this study, we have investigated how the retention and chromatographic separation of eight PEth homologues and the phospholipid background are influenced by changes in mobile phase composition using two different LC columns, the Acquity BEH C
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82e7e714979a77b706382c1d7a72ac5d https://doi.org/10.1016/j.chroma.2022.463566 Zobrazit plný text záznamu Full Text from ScienceDirect