| Autor: |
Paul Scheie |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Biochimica et Biophysica Acta (BBA) - General Subjects. 716:420-423 |
| ISSN: |
0304-4165 |
| DOI: |
10.1016/0304-4165(82)90035-6 |
| Popis: |
Lysozyme attacked Escherichia coli B/r in the absence of EDTA or imposed osmotic shocks when the cells were rapidly cooled below specific temperatures. Cells subjected to lysozyme while being cooled to below 20 degrees C began to lose ability to subsequently form colonies. This sensitivity increased with decreasing temperatures and almost all cells cooled to 0 degrees C were affected. Slightly hypertonic solutions did not improve survival. Cells cooled first to as low as 5 degrees C and then subjected to lysozyme while cool did not lose their ability to form colonies subsequent to rewarming. However, 70% of the cells cooled first to 0 degrees C and subjected to lysozyme lost their colony-forming ability. Cell lysis also began when treated near 5 degrees C, but even when treated at 0 degrees C about 50% of the cells maintained their rod shape in the presence of lysozyme. These results are discussed in terms of a possible phase transition in a portion of the cell envelope and/or a transient osmotic swelling as a results of metabolic pumps failing at the low temperatures. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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