Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain
| Autor: | Reeta Korhonen, Ismo Virtanen, Maciej Lalowski, Anne Rokka, Pascal Trippner, Jaana Tyynelä, Enzo Scifo, Sabine Koch, Garry L. Corthals, Maria Lindfors |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Proteome Blotting Western Cathepsin D Fluorescent Antibody Technique Biology ta3111 lcsh:RC321-571 Focal adhesion 03 medical and health sciences Myelin chemistry.chemical_compound Mice 0302 clinical medicine Cell Movement Neuronal Ceroid-Lipofuscinoses Gene expression medicine Animals Cluster Analysis Humans lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Cytoskeleton 030304 developmental biology Alpha-synuclein Mice Knockout 0303 health sciences Integrin assembly ta1182 Brain Computational Biology Cell projection organization Cell migration Fibroblasts medicine.disease Molecular biology Immunohistochemistry 3. Good health Cell biology medicine.anatomical_structure Neurology chemistry iTRAQ Focal adhesions Synapses Neuronal ceroid lipofuscinosis 030217 neurology & neurosurgery Cathepsin D deficiency |
| Zdroj: | Neurobiology of Disease, Vol 50, Iss, Pp 107-119 (2013) Neurobiology of Disease; Vol 50 Neurobiology of Disease |
| Popis: | Cathepsin D deficiency is a fatal neurodegenerative disease characterized by extreme loss of neurons and myelin. Our previous studies have demonstrated that structural and functional alterations in synapses are central to the disease pathogenesis. Therefore, we took a systematic approach to examine the synaptic proteome in cathepsin D knock-out mice, where the synaptic pathology resembles that of human patients. We applied quantitative mass spectrometry analysis on synaptosomal fractions isolated from cathepsin D knock-out and control mice at the age of 24 days. From the approximately 600 identified proteins, 43 were present in different amounts (P |
| Databáze: | OpenAIRE |
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