Neurons derived in vitro from ES cells express homeoproteins characteristic of motoneurons and interneurons

Autor: Serge Alonso, Patrick Carroll, Pierre Filippi, Yannick Renoncourt, Vilma Arce
Rok vydání: 1998
Předmět:
Central Nervous System
Embryology
PAX6 Transcription Factor
Tyrosine 3-Monooxygenase
Cellular differentiation
LIM-Homeodomain Proteins
Peripherins
Retinoic acid
Muscle Proteins
Nerve Tissue Proteins
Tretinoin
Biology
Choline O-Acetyltransferase
Nestin
Mice
chemistry.chemical_compound
Intermediate Filament Proteins
Interneurons
medicine
Animals
Paired Box Transcription Factors
Eye Proteins
Cells
Cultured
Homeodomain Proteins
Motor Neurons
Membrane Glycoproteins
Glutamate Decarboxylase
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
Gene Expression Regulation
Developmental
PAX7 Transcription Factor
Neural crest
Cell Differentiation
Peripherin
Embryonic stem cell
Molecular biology
DNA-Binding Proteins
Repressor Proteins
medicine.anatomical_structure
nervous system
chemistry
embryonic structures
Neuron
Stem cell
Biomarkers
Transcription Factors
Developmental Biology
Zdroj: Mechanisms of Development. 79:185-197
ISSN: 0925-4773
DOI: 10.1016/s0925-4773(98)00189-0
Popis: We have characterized different neuronal subpopulations derived from in vitro differentiation of embryonic stem (ES) cells using as markers the expression of several homeodomain transcription factors. Following treatment of embryo-like aggregates with retinoic acid (RA), Pax-6, a protein expressed by ventral central nervous system (CNS) progenitors is induced. In contrast, Pax-7 expressed in vivo by dorsal CNS progenitors, and erbB3, a gene expressed by neural crest cells and its derivatives, are almost undetectable. CNS neuronal subpopulations generated expressed combinations of markers characteristic of somatic motoneurons (Islet-1/2, Lim-3, and HB-9), cranial motoneurons (Islet-1/2 and Phox2b) and interneurons (Lim-1/2 or EN1). Molecular characterization of neuron subtypes generated from ES cells should considerably facilitate the identification of new genes expressed by restricted neuronal cell lineages.
Databáze: OpenAIRE
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