Crystallization and preliminary X-ray diffraction data for the carboxylesterase Est30 fromBacillus stearothermophilus
| Autor: | Chung-Dar Lu, Irene T. Weber, Yuan-Fang Wang, Hosam E. Ewis, Ahmed T. Abdelal, Ping Liu |
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| Rok vydání: | 2003 |
| Předmět: |
Diffraction
Ammonium sulfate Temperature General Medicine Recombinant Proteins Carboxylesterase law.invention Geobacillus stearothermophilus Kinetics chemistry.chemical_compound Crystallography X-Ray Diffraction chemistry Ammonium Sulfate Structural Biology law X-ray crystallography Molecule Crystallization Merge (version control) |
| Zdroj: | Acta Crystallographica Section D Biological Crystallography. 59:1472-1473 |
| ISSN: | 0907-4449 |
| DOI: | 10.1107/s0907444903011405 |
| Popis: | Crystals have been grown of the carboxylesterase Est30 from Bacillus stearothermophilus by hanging-drop vapor diffusion using ammonium sulfate as precipitant. The crystals diffracted to better than 2.0 A resolution. X-ray diffraction data were reduced in space group C222(1), with unit-cell parameters a = 55.83, b = 58.15, c = 179.65 A. R(merge) was 0.038 for 17 449 independent reflections with a completeness of 85.1%. V(M) was calculated to be 2.43 A(3) Da(-1), which suggested that there was one molecule of Est30 in the asymmetric unit. These crystals are suitable for structure determination. |
| Databáze: | OpenAIRE |
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