Immunophenotyping of immune cell populations in the raccoon (Procyon lotor)
| Autor: | Regina Carlson, V. Molnar, Peter Wohlsein, Christina Puff, Wolfgang Baumgärtner, Franziska Heinrich, Thomas Scheibe, Nicole Jungwirth, Andrea Tipold, Ingo Spitzbarth, Katja von Dörnberg, Michael Böer |
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| Rok vydání: | 2015 |
| Předmět: |
Myeloid
Lymphoid Tissue Immunology Biology Immunophenotyping Immune system Antigen Antigens CD parasitic diseases MHC class I medicine Leukocytes Animals Antigens Cloning Molecular Histiocyte General Veterinary Flow Cytometry Virology medicine.anatomical_structure Lymphatic system biology.protein Raccoons Antibody |
| Zdroj: | Veterinary immunology and immunopathology. 168(3-4) |
| ISSN: | 1873-2534 |
| Popis: | The raccoon (Procyon lotor) is a highly adaptable carnivore that has rapidly conquered Europe over the last decades and represents a potential candidate as pathogen reservoir, bearing the risk for transmission of infectious agents, as zoonosis or spill-over, to other wild life and domestic animals and man. Comprehensive investigations of infectious diseases in raccoons require a detailed knowledge of the participating immune cell populations. To close this gap of knowledge, various antibodies were tested for cross-reactivity with leukocytes in lymphoid organs and peripheral blood of raccoons using immunohistochemistry and flow cytometry, respectively. Eight out of 16 antibodies, directed against CD3, CD79α, Pax-5, IgG, CD44, MHC class II, myeloid/histiocyte antigen (MAC387), and Iba-1 exhibited a specific immunoreaction with cells in distinct anatomical compartments in formalin-fixed paraffin-embedded lymphoid tissues. Flow cytometric analysis revealed that 7 out of 18 antibodies directed against CD11c, CD14, CD21, CD44, CD79α, MHC class I and II cross-reacted with peripheral blood-derived raccoon leukocytes. Summarized, the usefulness of several cross-reacting antibodies was determined for the characterization of raccoon immune cells in immunohistochemistry and flow cytometry, offering the opportunity to study the raccoon immune system under normal and diseased conditions. |
| Databáze: | OpenAIRE |
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