In vitro selenium bioaccessibility combined with in vivo bioavailability and bioactivity in Se-enriched microalga (Chlorella sorokiniana) to be used as functional food
| Autor: | Ana Arias Borrego, Tamara García-Barrera, Inés Garbayo-Nores, Veronica Gómez-Jacinto, Carlos Vílchez-Lobato, Francisco Navarro-Roldán |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Bioavailability Se-enriched microalgae Speciation Medicine (miscellaneous) chemistry.chemical_element Urine Chlorella sorokiniana Excretion 03 medical and health sciences 0404 agricultural biotechnology Functional food In vivo 23 Química Mus musculus TX341-641 Bioaccesibility Food science 030109 nutrition & dietetics Nutrition and Dietetics Chemistry Nutrition. Foods and food supply 04 agricultural and veterinary sciences 040401 food science In vitro Selenium Food Science |
| Zdroj: | Journal of Functional Foods, Vol 66, Iss, Pp 103817-(2020) Arias Montano. Repositorio Institucional de la Universidad de Huelva instname |
| ISSN: | 2015-6790 |
| DOI: | 10.1016/j.jff.2020.103817 |
| Popis: | Speciation of seleno-metabolites and selenoproteins in Se-enriched Chlorella sorokiniana microalgae, in vitro and in vivo extracts has been performed by HPLC-ICP-MS. Selenium bioaccesibility in Se-enriched Chlorela sorokiniana evaluated by in vitro gastrointestinal digestion of the selenized microalga was 81% (79% as SeMet). Mice supplemeted with Se-enriched C. sorokiniana presented increased Se concentration in kidney, reflecting a potencial mechanism of excretion by urine. In addition, selenium bioavailavility, which was in the range of 3–15% depending on the diet was 1.13-fold higher in mice fed with Se-enriched microalgae against the basal diet, but decreased with higher amounts of selenium. Regarding bioactivity (∑Se as selenoproteins in serum/total Se in serum = 76–85% depending on the diet), selenium and selenoproteins (SeP) increased in serum from animals fed high supplemental Se levels. These results showed that Se-enriched algae can be considered as an alternative selenized food for humans, due to their high Se bioavailability. This work has been supported by the projects CTM2015-67902-C2-1-P and PG2018-096608-B-C21 from the Spanish Ministry of Economy and Competitiveness and P12-FQM-0442 and P09-FQM-04659 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). Finally, authors are grateful to FEDER (European Community) for financial support, grants number UNHU13-1E-1611 and UNHU15-CE-3140. |
| Databáze: | OpenAIRE |
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