Effect of association of non-steroidal anti-inflammatory and antibiotic agents with calcium hydroxide pastes on their cytotoxicity and biocompatibility
| Autor: | Marco Antonio Hungaro Duarte, Guilherme Ferreira da Silva, Francine Cesário, Rodrigo Ricci Vivan, Paulo Henrique Weckwerth, Rodrigo Cardoso de Oliveira, Alan Matheus Ramos Garcia |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diclofenac
Biocompatibility medicine.drug_class Pharmacology Anti-inflammatory Calcium Hydroxide Root Canal Filling Materials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Viability assay Cytotoxicity General Dentistry Calcium hydroxide Root Canal Irrigants Chemistry Anti-Inflammatory Agents Non-Steroidal 030206 dentistry Diclofenac Sodium Anti-Bacterial Agents Rats medicine.anatomical_structure CITOTOXICIDADE IMUNOLÓGICA 030220 oncology & carcinogenesis Subcutaneous tissue medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1436-3771 1432-6981 |
| DOI: | 10.1007/s00784-019-02923-y |
| Popis: | Evaluate the cytotoxicity and biocompatibility of calcium hydroxide paste [Ca (OH)2] associated with 5% diclofenac sodium, ibuprofen, or amoxicillin. Pre-osteoblast-like cells were cultivated and the MTT test was used to determine the cytotoxicity of the paste extracts after time intervals of 24, 48, 72 h, and 7 days. Tubes containing Ca (OH)2 pastes associated with the drugs and empty tubes were implanted in subcutaneous tissue of 30 rats. After 7 and 30 days, the specimens were removed and submitted to histological analysis. The data obtained were submitted to Kruskal-Wallis and Dunn tests (p ≤ 0.05). All the Ca (OH)2 pastes promoted cell viability after all periods. At 7 days, there was greater inflammatory tissue reaction adjacent to the implants. At 30 days, there was a significant reduction in the number of inflammatory cells, and increase in fibroblasts in all groups. In this period, a lower number of inflammatory cells and a higher number of fibroblasts were observed in the capsules adjacent to the association with diclofenac, when compared with the other mixtures (p ≤ 0.05); the capsule thickness was greater at 7 days than at 30 days. The Ca (OH)2 pastes associated with the drugs were not cytotoxic and presented biocompatibility after implantation in rat subcutaneous tissues. Ca (OH)2 pastes with anti-inflammatory or antibiotic may be clinical alternatives as intracanal medication to reduce resistant microorganisms in root canal system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink