Teaching Single-Cell Digital Analysis Using Droplet-Based Microfluidics

Autor:	Michael Ryckelynck, Majdi Najah, Andrew D. Griffiths
Rok vydání:	2012
Předmět:	Chemistry Microfluidics Droplet-based microfluidics Digital analysis Nanotechnology Hardware_ARITHMETICANDLOGICSTRUCTURES Analytical Chemistry
Zdroj:	Analytical Chemistry. 84:1202-1209
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac202645m
Popis:	Microfluidics allows the manipulation of small quantities of reagents in a high-throughput manner and is therefore highly amenable to single cell characterization and more generally to digital analysis, with applications in fields as varied as genomics, diagnostics, directed evolution, and drug screening. The growing place of microfluidics in biology laboratories encouraged us to develop a teaching method where advanced undergraduate or first-year graduate-level students are taught to fabricate droplet-based microfluidic devices, characterize them, and finally use them to perform a digital analysis of bacterial samples based on a phenotypic marker.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82dd47a2d2ae487202c4d7c81dc5df35 https://doi.org/10.1021/ac202645m Zobrazit plný text záznamu