Thrombospondin-1 is a glucocorticoid responsive protein in humans
| Autor: | Brenda L. Mangelsdorf, Warrick J. Inder, Jonathan P. Whitehead, Sahar Keshvari, Johannes B. Prins, Campbell H. Thompson, Johanna L. Barclay, Carolyn J. Petersons, Jane Sorbello, Morton G. Burt |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty Hydrocortisone Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Biology Sensitivity and Specificity Dexamethasone Thrombospondin 1 03 medical and health sciences Young Adult 0302 clinical medicine Endocrinology Glucocorticoid receptor Internal medicine Gene expression medicine Humans Cushing Syndrome Glucocorticoids Aged Aged 80 and over Matricellular protein General Medicine Middle Aged In vitro Cross-Sectional Studies 030220 oncology & carcinogenesis Female Glucocorticoid Biomarkers medicine.drug Adrenal Insufficiency |
| Zdroj: | European journal of endocrinology. 174(2) |
| ISSN: | 1479-683X |
| Popis: | ObjectiveThrombospondin-1 (TSP1) is a matricellular protein whose gene expression has previously been shown to increase acutely after exposure to dexamethasone in vitro. The aim of this study was to determine if TSP1 is altered by acute and chronic states of glucocorticoid excess in human subjects.Design and methodsThree studies have been undertaken to assess the difference or change in TSP1 in response to altered glucocorticoid activity: i) an acute interventional study assessed the effects of a single 4 mg dose of dexamethasone in 20 healthy volunteers; ii) a cross-sectional study compared plasma TSP1 in 20 healthy volunteers and eight patients with Cushing's syndrome; iii) an interventional study assessed the effect on plasma TSP1 of an increase in hydrocortisone dose from ≤20 mg/day to 30 mg/day for 7 days in 16 patients with secondary adrenal insufficiency.ResultsIn healthy volunteers, 4 mg dexamethasone significantly increased peripheral blood mononuclear cell (PBMC) TSP1 mRNA levels (PPP400 ng/ml diagnosed Cushing's syndrome with sensitivity of 100% and specificity of 85%. The higher hydrocortisone dose increased plasma TSP1 from 139 (86–199) to 256 (133–516) ng/ml, (PConclusionsTSP1 is a glucocorticoid responsive protein in humans. Further research is required to determine if plasma TSP1 has a role as a glucocorticoid biomarker. |
| Databáze: | OpenAIRE |
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