Tailored coating of gold nanostars: rational approach to prototype of theranostic device based on SERS and photothermal effects at ultralow irradiance
| Autor: | S Perversi, Angelo Taglietti, Alessia Pascale, Pietro Galinetto, Giacomo Dacarro, Piersandro Pallavicini, Nicoletta Marchesi, Barbara Bassi, E. Giulotto |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Bioengineering Nanotechnology 02 engineering and technology Polyethylene glycol engineering.material 010402 general chemistry 01 natural sciences HeLa chemistry.chemical_compound Coating General Materials Science Electrical and Electronic Engineering biology Mechanical Engineering Photothermal effect General Chemistry Photothermal therapy 021001 nanoscience & nanotechnology Biocompatible material biology.organism_classification 0104 chemical sciences Membrane chemistry Mechanics of Materials Cancer cell engineering 0210 nano-technology |
| Zdroj: | Nanotechnology. 29:235301 |
| ISSN: | 1361-6528 0957-4484 |
| Popis: | The last decade has come across an increasing demand for theranostic biocompatible nanodevices possessing the double ability of diagnosis and therapy. In this work, we report the design, synthesis and step-by-step characterization of rationally coated gold nanostars (GNSs) for the SERS imaging and photothermal therapy of HeLa cancer cells. The nanodevices were realized by synthesizing GNSs with a seed growth approach, coating them with a controlled mixture of thiols composed of a Raman reporter and a polyethylene glycol with a terminal amino group, and then reacting these amino groups with folic acid (FA), in order to impart selectivity towards cancer cells which overexpress folate receptors on their membranes. After a complete characterization, we demonstrate that these FA-functionalized GNSs (FA-GNSs) are able to bind selectively to the membranes of HeLa cells, acting as SERS tags and allowing SERS imaging. Moreover, we demonstrate that once bound to HeLa cell membranes, FA-GNSs exhibit photothermal effect which can be exploited to kill the same cells in vitro using laser irradiation in the NIR at a very low and safe irradiance. We thus demonstrate that the FA-GNSs designed following the described approach are an efficient prototype of theranostic nanodevices. |
| Databáze: | OpenAIRE |
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