Isolation of calcium-transporting lipid from the mitochondrial glycolipoprotein
| Autor: | Yagushinskij Ls, Evtodienko JuV, Tamara S Azarashvily, Luk'yanenko Ai, Medvedev Bi |
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| Rok vydání: | 1982 |
| Předmět: |
Membrane lipids
Lipoproteins Clinical Biochemistry Lipid Bilayers Biological Transport Active Mitochondria Liver Membrane Potentials Membrane fluidity Animals Lipid bilayer phase behavior Lipid bilayer Molecular Biology Lipid raft Glycoproteins Adenosine Triphosphatases Chemistry Bilayer Peripheral membrane protein Electric Conductivity Biological membrane Cell Biology General Medicine Lipids Rats Molecular Weight Proton-Translocating ATPases Biochemistry lipids (amino acids peptides and proteins) Calcium |
| Zdroj: | Molecular and cellular biochemistry. 48(1) |
| ISSN: | 0300-8177 |
| Popis: | From the mitochondrial Ca2+-transporting glycolipoprotein (GLP) the lipid was isolated which induced Ca2+-translocation through bilayer lipid membranes. Electroconductivity of modified phospholipid membranes in the presence of CaCl2 is increased 150-200 times. At 10-fold CaCl2 gradient a generation of membrane potential is observed close to its theoretical value. It is shown that the lipid forms separate conductivity channels of 10 and 20 pS in the bilayer. The mode of action of GLP in the membrane is proposed. It is assumed that the carbohydrate part of GLP is a selective receptor-accumulator for Ca2+, whereas the function of the lipid component consists in forming channels in the bilayer. |
| Databáze: | OpenAIRE |
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