What is pure hemozoin? A close look at the surface of the malaria pigment
| Autor: | E. Danae Guerra, D. Scott Bohle, Marta Cerruti, Elias Georges, Fadi Baakdah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Hemeproteins
Surface Properties Plasmodium falciparum Analytical chemistry 010402 general chemistry 01 natural sciences Biochemistry Inorganic Chemistry chemistry.chemical_compound Adsorption X-ray photoelectron spectroscopy Desorption Ionization time of flight Carboxylate Amino Acids Malaria pigment chemistry.chemical_classification 010405 organic chemistry Photoelectron Spectroscopy Hemozoin Biomolecule Erythrocyte Membrane 3. Good health 0104 chemical sciences chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Crystallization |
| Zdroj: | Journal of Inorganic Biochemistry. 194:214-222 |
| ISSN: | 0162-0134 |
| DOI: | 10.1016/j.jinorgbio.2019.01.021 |
| Popis: | The malaria parasite, Plasmodium spp., produces hemozoin (Hz) crystals as a by-product of hemoglobin digestion. Purification methods used to remove host or parasite products adsorbed on Hz surface lead to variable and undetermined residues. This compositional variation likely accounts for the assortment of contradictory results in studies of Hz's biomineralization, immunomodulating properties, and the mechanism of action of some antimalarials. In this work, we study the surface of Hz cleaned with two methods, both reported in the literature, one stricter than the other. We find that biomolecules are adsorbed on Hz treated with either method, they bind through carboxylate groups, and may be present within Hz structure. Their composition and amount depend on the washing protocol, which also introduces contaminants. This finding led us to question the concept of "pure" Hz, and to propose x-ray photoelectron spectroscopy (XPS) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) as characterization tools to assess surface contamination prior to further work on Hz crystals. |
| Databáze: | OpenAIRE |
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