Angiogenic effects of long-term enhanced external counterpulsation in a dog model of myocardial infarction
| Autor: | Dian-qiu Fang, Guifu Wu, William Lawson, Khan Tanveer Ahmed, Zhensheng Zheng, Cheng-hen Hu, John C.K. Hui, Hong Ma, Roger J. Laham, Zhimin Du, Cheng-yang Zhan |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Physiology Myocardial Infarction Gene Expression Neovascularization Physiologic Infarction Hemodynamics Enhanced external counterpulsation Coronary artery disease Dogs Counterpulsation Physiology (medical) Internal medicine Animals Medicine Myocardial infarction Ligation business.industry Myocardium medicine.disease Collateral circulation Coronary Vessels Disease Models Animal Coronary occlusion Cardiology Cardiology and Cardiovascular Medicine business |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 290:H248-H254 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.01225.2004 |
| Popis: | Enhanced external counterpulsation (EECP) is an effective noninvasive treatment of coronary artery disease. Its mechanism of action remains unknown. An acute coronary occlusion dog model was created to explore the angiogenic effect of EECP. After coronary occlusion, 12 dogs were randomly assigned to either EECP ( n = 6) or control ( n = 6). Immunohistochemical studies of α-actin and von Willebrand factor (vWF) were used to detect newly developed microvessels. Systemic and local vascular endothelial growth factor (VEGF) were identified by ELISA and reverse transcriptase PCR analysis. There was a significant increase in the density of microvessels per squared millimeter in the infarcted regions of the EECP group compared with the control group (vWF, 15.2 ± 6.3 vs. 4.9 ± 2.1, P < 0.05; α-actin, 11.8 ± 5.3 vs. 3.4 ± 1.2, P < 0.05). The positive-stained area per squared micrometer also increased significantly (α-actin, 6.6 × 103 ± 2.9 × 103 μm2 vs. 0.6 × 103 ± 0.5 × 103 μm2, P < 0.05; vWF, 5.7 × 103 ± 1.9 × 103 μm2 vs. 1.7 × 103 ± 1.4 × 103 μm2, P < 0.05). Immunohistochemical staining and reverse transcriptase PCR analysis documented a significant increase in VEGF expression. These factors associated with angiogenesis corresponded to improved myocardial perfusion by 99mTc-sestamibi single-photon emission computed tomography. Angiogenesis may be a mechanism of action for the improved myocardial perfusion demonstrated after EECP therapy. |
| Databáze: | OpenAIRE |
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