Relative potencies of polychlorinated dibenzo-p-dioxins (PCDDS), dibenzofurans (PCDFS) and biphenyls (PCBS), for cytochrome P450 1A induction in the mirror carp (Cyprinus carpio)
| Autor: | Martin van den Berg, Martine E.J. van der Weiden, Willem Seinen, Luite Peter de Vries, K.M. Fase, Malin Celander |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Stereochemistry Health Toxicology and Mutagenesis biology.animal_breed Aquatic Science Oceanography Cyprinus Internal medicine medicine Enzyme inducer Carp ED50 Mirror carp biology Chemistry Potent inducer Cytochrome P450 General Medicine biology.organism_classification Pollution Fishery Congener Endocrinology Polychlorinated Dibenzo-p-dioxins Toxicity biology.protein |
| Zdroj: | Aquatic Toxicology. 29:163-182 |
| ISSN: | 0166-445X |
| DOI: | 10.1016/0166-445x(94)90066-3 |
| Popis: | Carp received a single i.p. dose of 2,3,7,8-TCDD, 2,3,7,8-TCDF, 1,2,3,7,8-PnCDD, 2,3,4,7,8-PnCDF, 1,2,3,6,7,8,-HxCDD, OCDF, OCDD, 2,3′,4,4′,5-PCB (PCB 118), 3,3′,4,4′,5-PCB (PCB126) or 2,2′,4,4′,5,5′-PCB (PCB 153). Four doses per congener were used increasing by a factor five. Fish were sacrificed after 2 weeks and hepatic cytochrome P450 1A protein content (P450 1A), 7-ethoxyresorufin-O-deethylation (EROD) activity and hepatic concentrations of the inducing compounds were determined. The aim of this study was to determine the relative, P450 1A-inducing potencies (RP) of several PCDDs, PCDFs and PCBs, relative to 2,3,7,8-TCDD. RPs were calculated based on ED50s derived from the administered doses or hepatic concentrations for induction of EROD or P450 1A protein levels. The RPs (molar ED50 TCDD/molar ED50 congener), based on EROD induction and internal concentration, for 2,3,7,8-TCDF, 1,2,3,7,8-PnCDD, 2,3,4,7,8-PnCDF and 1,2,3,6,7,8-HxCDD were 0.03, 0.68, 0.36 and 0.59, respectively. The RPs of PCB 118 and PCB 126 were estimated to be 0.0004 and > 0.005, respectively. No RP could be calculated for OCDD and PCB 153, due to the absence of P450 1A induction by these congeners. PnCDD appeared to be the most potent inducer of hepatic P450 1A. A statistically significant EROD induction was already effected by a concentration of 63.75 pg PnCDD/g liver, which was in the present study the lowest effective concentration of all congeners. |
| Databáze: | OpenAIRE |
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