Hepatitis B virus genomic sequence in the circulation of hepatocellular carcinoma patients: comparative analysis of 40 full-length isolates
| Autor: | Kazuaki Takahashi, K. Hino, Y. Ohta, Y. Akahane, Shunji Mishiro |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Hepatitis B virus HBsAg Carcinoma Hepatocellular Molecular Sequence Data Genome Viral medicine.disease_cause Genes env Polymerase Chain Reaction Orthohepadnavirus Virology Genotype medicine Humans Missense mutation Amino Acid Sequence Promoter Regions Genetic Phylogeny Aged DNA Primers Mutation Hepatitis B Surface Antigens Base Sequence biology Viral Core Proteins Liver Neoplasms General Medicine Middle Aged Hepatitis B medicine.disease biology.organism_classification digestive system diseases Hepadnaviridae Hepatocellular carcinoma DNA Viral Female |
| Zdroj: | Archives of Virology. 143:2313-2326 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | We determined full-length nucleotide sequence of hepatitis B virus (HBV) genome in sera from 40 Japanese patients with HBsAg-positive hepatocellular carcinoma (HCC), in order to obtain information on HCC-specific characteristics, if any, of the HBV genome. Direct sequencing of the long distance PCR products starting from 50 microliters of serum samples revealed that 95% of our isolates were of genotype C, and that mutations and deletions/insertions were very common. With respect to envelope protein genes, deletions and missense mutations were frequent in preS2, and the determinant a domain of HBsAg was rich in "antibody-escape" mutations. Within the precore/core region, the most remarkable mutation was the replacement of proline of wild type by other amino acids at codon 130 of the core gene, which was found in 58% of our isolates, while precore-stop mutation was found in 45%. Most interestingly, however, about 90% of our isolates had mutations at nt positions 1762 (A-to-T) and 1764 (G-to-A) within the core promoter, which had been implicated in "e-suppressive" phenotype of HBV genome. G-to-A at nt 1613 and C-to-T at nt 1653 within enhancer II and T-to-C/A at nt 1753 within core promoter were also evident: 38%, 53%, and 40%, respectively. It was interesting that some of the characteristics observed in our isolates form HCC patients had been previously implicated in fulminant hepatitis and/or acute exacerbation of chronic hepatitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink