High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains
| Autor: | Dara S. Lo, Brenda J. Andrews, Ben T. Grys, Mojca Mattiazzi Usaj |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Microscopy Confocal Saccharomyces cerevisiae Proteins Chemistry Mutant High throughput imaging Saccharomyces cerevisiae Yeast Article law.invention Cell biology High-Throughput Screening Assays 03 medical and health sciences 0302 clinical medicine Microscopy Fluorescence Confocal microscopy law High-content screening Microscopy Mutation Fluorescent protein 030217 neurology & neurosurgery 030304 developmental biology Fluorescent Dyes |
| Zdroj: | Methods Mol Biol Confocal Microscopy ISBN: 9781071614013 |
| ISSN: | 1940-6029 |
| Popis: | We describe a protocol for live-cell high-throughput (HTP) screening of yeast mutant strains carrying fluorescent protein markers for subcellular compartments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This HTP protocol is readily adapted for screening any combination of markers and can be expanded to different growth conditions or higher order mutant genetic backgrounds. |
| Databáze: | OpenAIRE |
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