Anti-proliferative activity of a purified polysaccharide isolated from the basidiomycete fungus Paxillus involutus
| Autor: | Wai Ming Li, Ankush Barad, Linda E. Tackaberry, Chow H. Lee, Bryan Chu Chwen Lim, Xiaojie Li, Keith N. Egger, Faran Rashid, Kerry B. Reimer, Hugues B. Massicotte, Sebastian Mackedenski, Peter C.K. Cheung |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Polymers and Plastics Mannose Apoptosis Xylose Chemical Fractionation Complex Mixtures Polysaccharide Fucose Gas Chromatography-Mass Spectrometry Microbiology 03 medical and health sciences chemistry.chemical_compound Polysaccharides Cell Line Tumor Materials Chemistry Humans Paxillus involutus Phylogeny Cell Proliferation chemistry.chemical_classification Mushroom biology Basidiomycota Organic Chemistry Monosaccharides 030108 mycology & parasitology biology.organism_classification 030104 developmental biology Biochemistry chemistry Galactose Growth inhibition |
| Zdroj: | Carbohydrate polymers. 181 |
| ISSN: | 1879-1344 |
| Popis: | A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229 kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC–MS methylation analysis suggests that GIPinv has mixed linkages in the backbone containing (1 → 6)-Gal (25.5%), (1 → 4)-Glc (18.3%), (1 → 6)-Glc (8.3%), (1 → 3)-Glc (5.3%) and (1 → 2)-Xyl (4.5%). GIPinv has branching points at (1 → 2, 6)-Man (8.6%) and (1 → 3, 6)-Man (4.9%) having unsubstituted fucose (8.3%) and glucose (16.3%) as terminal sugars. GIPinv had growth-inhibitory activity against several cancer cell lines and triggered apoptosis. GIPinv should be further explored as a potential anti-cancer agent and a unique polysaccharide. |
| Databáze: | OpenAIRE |
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