Spodoptera frugiperda resistance to oral infection by Autographa californica multiple nucleopolyhedrovirus linked to aberrant occlusion-derived virus binding in the midgut
| Autor: | Eric J. Haas-Stapleton, Jan O. Washburn, Loy E. Volkman |
|---|---|
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Journal of General Virology. 86:1349-1355 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.80845-0 |
| Popis: | Spodoptera frugiperda larvae are highly resistant to oral infection by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) (LD50, ∼9200 occlusions), but extremely susceptible to budded virus within the haemocoel (LD50, S. frugiperda midgut cells contributes to lack of oral infectivity, the binding and fusion properties of AcMNPV ODV were compared with those of the ODV of a new isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) obtained from a field-collected larva (oral LD50, 12 occlusions). By using a fluorescence-dequenching assay conducted in vivo, it was found that AcMNPV ODV bound to the midgut epithelia of S. frugiperda larvae at ∼15 % of the level of SfMNPV ODV, but that, once bound, the efficiencies of fusion for the two ODVs were similar: 60 % for AcMNPV and 53 % for SfMNPV. Whilst the difference in binding efficiencies was significant, it could not account entirely for the observed differences in infectivity. Competition experiments, however, revealed that, in S. frugiperda larvae, SfMNPV ODV bound to a midgut cell receptor that was not bound by AcMNPV ODV, indicating that ODV interaction with a specific receptor(s) was necessary for productive infection of midgut columnar epithelial cells. Fusion in the absence of this ligand–receptor interaction did not result in productive infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|