Protein profile analysis from advanced third-stage larvae (AdvL3) and adult worms of Gnathostoma binucleatum (Nematoda: Spirurida)
| Autor: | Miguel Ángel Mosqueda-Cabrera, María de Lourdes Caballero-García, Enedina Jiménez-Cardoso, Roberto Javier Almeyda-Artigas |
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| Rok vydání: | 2006 |
| Předmět: |
Trichinella spiralis
Immunoblotting Spirurida Infections Microbiology parasitic diseases Animals Humans Echinococcus granulosus Dipylidium caninum Gnathostoma General Veterinary biology Gene Expression Profiling fungi General Medicine Helminth Proteins biology.organism_classification Infectious Diseases Nematode Insect Science Larva Immunology Parasitology Female Ascaris lumbricoides Ancylostoma caninum Toxocara canis |
| Zdroj: | Parasitology research. 100(3) |
| ISSN: | 0932-0113 |
| Popis: | Proteins from crude extracts of advanced third-stage larvae and adult Gnathostoma binucleatum nematode worms showed protein profiles in SDS-PAGE analysis similar to Echinococcus granulosus, Trichinella spiralis, Dipylidium caninum, Ancylostoma caninum, Ascaris lumbricoides and Toxocara canis. The immunoblot analysis of the human serum infected or suspected to be infected with G. binucleatum using the total larvae extract recognized the 40, 60, 80 and 115 kDa proteins and using the total adult worm extract recognized only the 80 and 115 kDa proteins. However, the 115 kDa protein showed cross-reactions with A. caninum, A. lumbricoides, T. canis and D. caninum with human serum positive to gnathostomosis, while the 40 kDa protein was only recognized with the G. binucleatum total larvae extract. The results obtained suggest that the use of antigens from the advanced third-stage larvae of the parasite were best recognized for immunodiagnosis of gnathostomosis. |
| Databáze: | OpenAIRE |
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