PLK4 trans-Autoactivation Controls Centriole Biogenesis in Space
| Autor: | Maria E. Francia, Adán Guerrero, Inês Bento, Swadhin Chandra Jana, Sihem Zitouni, Paulo Duarte, Francisco Freixo, Samuel Gilberto, Mónica Bettencourt-Dias, Jorge Carneiro, Carla A.M. Lopes, Inês Cunha-Ferreira, Mariana Lince-Faria |
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| Rok vydání: | 2015 |
| Předmět: |
PLK4
Centriole sports Biology Protein Serine-Threonine Kinases General Biochemistry Genetics and Molecular Biology Procentriole Peroxisomes Basal body Animals Drosophila Proteins Phosphorylation Spermatogenesis Molecular Biology Centrioles Regulation of gene expression Centrosome Cilium Gene Expression Regulation Developmental Cell Biology Protein-Serine-Threonine Kinases Cell biology sports.league Drosophila melanogaster Biogenesis Developmental Biology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2015.09.020 |
| Popis: | The deposited article is a post-print version and has been submitted to peer review. This publication hasn't any creative commons license associated. The deposited article version contains attached the supplementary materials within the pdf. Centrioles are essential for cilia and centrosome assembly. In centriole-containing cells, centrioles always form juxtaposed to pre-existing ones, motivating a century-old debate on centriole biogenesis control. Here, we show that trans-autoactivation of Polo-like kinase 4 (PLK4), the trigger of centriole biogenesis, is a critical event in the spatial control of that process. We demonstrate that centrioles promote PLK4 activation through its recruitment and local accumulation. Though centriole removal reduces the proportion of active PLK4, this is rescued by concentrating PLK4 to the peroxisome lumen. Moreover, while mild overexpression of PLK4 only triggers centriole amplification at the existing centriole, higher PLK4 levels trigger both centriolar and cytoplasmatic (de novo) biogenesis. Hence, centrioles promote their assembly locally and disfavor de novo synthesis. Similar mechanisms enforcing the local concentration and/or activity of other centriole components are likely to contribute to the spatial control of centriole biogenesis under physiological conditions. Fundação Portuguesa para a Ciência e Tecnologia grants: (SFRH/BPD/87479/2012, PTDC/BBB-BEP/1724/2012, HMSP-CT/SAU-ICT/0075/2009, PTDC/SAU-OBD/105616/2008, EXPL/BIM-ONC/0830/2013, PTDC/BBB-BEP/1724/2012); EMBO installation grant; ERC starting grant: (PFE-GI-UE-ERC-2010-StG-261344). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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