Multiple in vivo pathways for E. coli small ribosomal subunit assembly occur on one pre-rRNA
| Autor: | Gloria M. Culver, Neha Gupta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Genetics
RNA Folding Eukaryotic Large Ribosomal Subunit fungi Ribosome biogenesis Ribosome Subunits Small Bacterial Biology Ribosomal RNA Article Cell biology 5S ribosomal RNA Ribonucleoproteins Structural Biology Ribosomal protein Ribosome Subunits RNA Ribosomal 16S parasitic diseases Escherichia coli RNA Precursors Eukaryotic Ribosome Molecular Biology Biogenesis |
| Zdroj: | Nature structural & molecular biology |
| ISSN: | 1545-9985 1545-9993 |
| Popis: | Processing of transcribed precursor ribosomal RNA (pre-rRNA) to a mature state is a conserved aspect of ribosome biogenesis in vivo. We developed an affinity-purification system to isolate and analyze in vivo-formed pre-rRNA-containing ribonucleoprotein (RNP) particles (rRNPs) from wild-type E. coli. We observed that the first processing intermediate of pre-small subunit (pre-SSU) rRNA is a platform for biogenesis. These pre-SSU-containing RNPs have differing ribosomal-protein and auxiliary factor association and rRNA folding. Each RNP lacks the proper architecture in functional regions, thus suggesting that checkpoints preclude immature subunits from entering the translational cycle. This work offers in vivo snapshots of SSU biogenesis and reveals that multiple pathways exist for the entire SSU biogenesis process in wild-type E. coli. These findings have implications for understanding SSU biogenesis in vivo and offer a general strategy for analysis of RNP biogenesis. |
| Databáze: | OpenAIRE |
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